Oral tissues acquire oxidative damage from exposure to free radicals generated by lifestyle choices, the environment and some dental procedures. In this study, hydrogen peroxide (H2O2), ethanol (EtOH) and nicotine (Nic) decreased cell viability/DNA synthesis and elevated reactive oxygen species (ROS) on cultured oral fibroblasts. The oral fibroblasts were then treated with bioactive antioxidant (AO) combinations — resveratrol, ferulic acid and tetrahydrocurcuminoid (RFT), phloretin, ferulic acid and resveratrol (PFR), phloretin, refulic acid and tetrahydrocurcuminoid (PFT) — counteracted by restoring DNA synthesis levels and cell viability, and decreasing the total ROS activity.
